µParaflo® Microarray Technology

Massively parallel oligonucleotide and peptide synthesis on a microchip based on the µParaflo® microfluidics technology

The only platform providing microarrays of oligonucleotide, peptide, and their analog sequences by in situ synthesis using conventional chemistry and a comprehensive list of off-the-shelf synthetic building blocks.

Our scientists and engineers have developed platform technologies that encompass a new class of microfluidic µParaflo® reaction devices, an advanced digital light synthesizer apparatus, and picoliter scale biochemical processes.  The functionalized µParaflo® chips are particularly suited for applications where small sample consumption, contamination-free, and performance-reproducibility are primary concerns. This technology enables the massively parallel synthesis of high quality DNA and RNA oligonucleotides as well as peptides and peptidomimetics in picoliter-scale reaction chambers.

  1. High Density – On Chip Parallel Synthesis.
  2. Customizable – DNA, RNA, Peptides, and Analogs
  3. Versatile – Uses Conventional Chemistry

  • The synthesis method is suitable for different classes of molecules, such as oligonucleotides, peptides, or their analogs.
  • The synthesis can be performed in a regular research laboratory.
  • The synthesis efficiency can be optimized to be comparable with conventional synthesis.
  • It offers flexibility in the sequences to be synthesized – editing the changes in sequence text files to create different chips easily.
  • The overall consumption of chemicals (in synthesis) and samples (in assays) is significantly reduced to the level of sub-nanoliter to picoliter per assay.
  • The microfluidic reaction chambers are particularly suited for parallel biochemical reactions.
  • The liquid delivery to the microchip is simple (parallel flow through the inlet and outlet holes), can be automated, and there is little chance of ambient contamination.
  • The spot density of the chip can be ten-fold higher than that of spotted chip.

Spotted Array

  • These are raw data.  Click the images to take a closer look.
  • Inset shows enlarged spots and intensity profiles through spots.
  • Color scheme: blue (0) to red (65000).
  • Intensity within each feature varies 3X (from 5000 to 15000) on the spotted array.
  • Intensity variation within each feature is negligible on the µParaflo® array.

µParaflo<sup>®</sup> microfluidic biochip