VariantPro™ Targeted Sequencing Technology
The VariantPro™ system is an innovative multiplex PCR based targeted sequencing method that accomplishes target selection, library preparation and molecular tagging in a simple one-step workflow.
One innovation in the VariantPro system is Relay-PCR, in which one pair of common (indexed library) primers and multiple pairs of specific primers are mixed with a genomic DNA sample in a single tube. A single PCR run seamlessly combines two functionally separated reaction phases, namely target replication and library amplification. This results in a significantly simplified work flow. Another innovation in the VariantPro system is Omega-Primer which is composed of three functional sections: a 5p arm designed to ensure stable binding to a corresponding template, a 3p arm designed to double check sequence specificity and initiate polymerase extension, and a separation segment between the two arms. The use of two separate binding sections provides primer design freedoms that balance priming specificity and binding strength.