LC Sciences provides a fast, one-stop Poly(A) RNA sequencing solution – from your sample through data analysis.

Poly(A) RNA-Seq is an advanced method for the quantification of gene expression in a biological sample under specific conditions, and enables the identification of novel transcripts, alternative splicing and gene fusion events to reveal a complete picture of the transcriptome.


  1. The latest in next-gen sequencing technology
  2. Experienced staff to assist you with experimental design
  3. Fast turnaround times
  4. Full bioinformatics analysis
  5. Expert technical support to assist with data interpretation


Library Preparation

Next-Gen Sequencing

Advanced Data Analysis

Any Species

Profile mRNA in any species – there is no need for probes based on prior sequence or secondary structure information.

Transcriptome-wide

Profiling is transcriptome-wide – investigate all mRNAs, known and unknown, in your sample.

Digital Results

Achieve digital, quantitative transcript expression analysis – Accurate expression levels over 5 orders of magnitude.

Novel Transcripts

Detect novel and rare transcripts and transcript variants, such as single nucleotide mutations (SNP)

  1. Alignment, classification, & functional annotation of all mapped reads
  2. Biostatistical analysis – expression analysis, multi-parameter data analysis, length distribution, transcript copy number comparisons, etc
  3. Prediction of novel transcripts
  4. GO and KEGG annotation and enrichment analysis
  5. Statistics and annotation of SNPs/Indels
  6. Alternative splicing analysis

Sample Data

Sample Requirements

Total RNA amount ≥ 2 μg
260/280 >1.8, 260/230 >1.0
RIN value ≥ 7.0

Library
Preparation

Poly(A) Tail RNA Selection
250~300 bp Insert cDNA Library

Recommended
Sequencing Depth

150 bp Paired End
>40 Million Reads

High Data Quality

Data quality is measured by the percentage of bases with a sequencing quality score above Q30.

These comprehensive services are designed to be one-stop and produce the results needed to quickly advance your biological and biomedical research. For all types of RNA sequencing, our “Total RNA to Data” services include: sample prep/QC, library preparation, RNA sequencing, bioinformatics analysis, and high-level customer support.

LC Sciences has been providing RNA discovery, profiling and related bioinformatics services since 2005 and our experts have examined thousands of varied RNA datasets, giving us unique insight into RNA research. We don’t merely deliver data; we will handle all the advanced bioinformatics analysis and help you find answers to the questions you’re asking.