Through the use of high density arrays (PepArray™) with addressable “signature” peptides for specific kinds of protein binding domains we can systematically screen thousands of sequences in a single experiment.  A proprietary microarray platform and advanced microfluidic technologies ensure quantitative measurements of binding events.

This combination of high-throughput capacity with quantitative measurement enables us to quickly and efficiently identify high affinity and high specificity target binding compounds and detect the presence or absence of these kinds of proteins in complex biological samples (cell lysate, human serum).

This technology is made available through our comprehensive phosphopeptide binding – peptide microarray service.

This comprehensive service includes assistance with your sequence designs, binding assays using sample(s) provided by you, single or dual color detection of the binding using a suitable method, image data processing, and signal data processing. Two-four weeks after receiving your sample(s), we send you a data summary report containing background subtraction, control and reference signal guided data processing, list of detected signals, and data averaging. We offer additional services for in-depth binding data analysis and curve analysis of quantitative measurements.

The published work describes the use of our phosphopeptide microarray to explore the possibility of identifying phosphoproteins that are pivotal in eliciting cellular responses during the process of normal cells becoming cancerous. These phosphoproteins have the potential as biomarkers to detect early cellular responses as a signature of cancer initiation and progression. (read more…)

Reference
Krishnamoorthy S, Liu Z, Hong A, Zhu R, Chen H, Li T, Zhou X, Gao X. (2013) A Novel Phosphopeptide Microarray Based Interactome Map in Breast Cancer Cells Reveals Phosphoprotein-GRB2 Cell Signaling Networks. PLoS ONE 8(6), [abstract]

Phosphopeptide binding arrays

We have standard content phosphopeptide binding arrays with information from literature incorporated into the standard array design.  Important signaling protein binding domains are represented on the array.

Our proteomics services include the use of on-chip synthesized peptide microarrays based on the PepArray™ technology which enables the total customization of content on each individual microarray to suit your needs.  Our comprehensive services includes sequence retrieval from public databases and literature and/or assistance with your sequence designs.

PepArray pro is a proteomics tool to provide PepArray Layout file that contains information about peptides, peptide IDs, and the array-location of the peptides to be synthesized on chip. PepArray pro supports the generation of peptide sequences containing standard or non-standard amino acids from reading user-input sequences, importing from web resources, or modifying existing peptides.  Currently, phosphopeptides from the corresponding databases Phospho.ELM and PepCyber P~PEP are also supported. The designed Layout file can incorporate reference and/or control peptides for quality of synthesis and assay, generate peptide modifications, replicate the generated peptides, and provide design statistics.  The designed PepArrays can be stored and archived.  PepArray pro makes available of a set of catalog PepArray Layout files.

  1. Assay SHP2 and SH2 Proteins
  2. Pathway studies
  3. Phosphotyrosine binding protein (PTB) studies
  4. Differential binding affinity analysis
  5. Quantitatively measurement of inhibitor effect
  6. Peptidomimetics Studies

This is a comprehensive phosphopeptide binding – peptide microarray service.  Just send us your protein sample and we’ll perform all the necessary functions from sample assay to data analysis. Our phosphopeptide binding comprehensive service includes: assistance with your sequence designs, synthesis of a custom phosphopeptide binding – peptide microarray, all on-chip binding reactions, detection of the bound protein using a suitable method, array image scan, and data analysis.  We offer additional services for in-depth binding data analysis and curve analysis of quantitative measurements.

Measurement of phosphopeptide binding on a peptide microarray offers the opportunity to study thousands (up to tens of thousands of peptides/chip) of specific binding sequences in a single experiment. One experiment using our 4K phosphopeptide binding – peptide microarray is equivalent to 41 experiments using a 96-well plate.  A 30K microarray format is also available.

Peptides are synthesized on-chip, not pre-synthesized and spotted.  There is no up-front cost to pre-synthesize a peptide library.  We can synthesize a completely custom one-of-a-kind peptide microarray specifically for your experiment delivering results that cannot be achieved with an off-the-shelf assay.  With the ability to program new sequence designs on the fly, you can quickly revise microarray design and content to keep experiments moving forward based on previous results.

Microfluidic phosphopeptide binding – peptide microarrays using our PepArray™ technology produce more uniform spots and enhanced binding kinetics to deliver a more reliable reading of the assay signals. Well designed positive and negative controls as well as multiple replicates of each peptide sequence contribute to data confidence.