LC Sciences offers a customized solution to high-throughput genome wide variant analysis. The VariantPro system is an innovative amplicon-based targeted sequencing technology for variant discovery and detection.

Examining the sequence variants of a targeted number of relevant genes and relevant metabolic pathway genes focuses the power of next-gen sequencing (NGS) and makes routine testing and data analysis feasible. Multiplexing polymerase chain reaction (PCR) is a proven, simple method to extract target regions of interest.

LC Sciences has developed the underlying technologies for an amplicon-based targeted sequencing system engineered to accomplish all the required steps for accurate NGS testing (target selection, library preparation and molecular tagging) in a simple one-step workflow. Made possible by the novel and patent pending Relay-PCR™ and Omega-Primer™ technologies, the VariantPro targeted sequencing system offers cost-effective, ultra-high resolution sequence analysis that enables accurate detection of rare variants. The flexibility and scalability of the technology means a custom assay can be tailored to fit a wide array of applications based on your specific needs.

  1. Discover germline or somatic mutations (e.g., cancer and disease associated regions)
  2. Detect and quantify rare transcripts and transcript variants, such as single nucleotide mutations (SNP)
  3. Detect low frequency alleles
  4. Follow-up studies from whole genome approaches to validate mutations
  5. Resequence large custom genomic regions
  6. High throughput genotyping

VariantPro Technology
VariantPro  accomplishes target selection, library preparation and molecular tagging in a simple one-step workflow.

LC Sciences offers a customized solution to high-throughput genome wide variant analysis. Our in-house developed VariantPro method incorporates novel Relay-PCR and Omega Primer technologies to produce amplicons of high uniformity & specificity.  State of the art Illumina sequencing technology is the most successful and widely adopted next-generation sequencing (NGS) technology worldwide.

This service is comprehensive – from sample to data, providing advanced technology and years of experience.

Our “Sample to Data” comprehensive service includes: primer designs, sample QC, sample preparation (target selection, sample bar-coding, molecular tagging, library amplification), high-throughput sequencing, bioinformatics analysis, and customer data report including:

  • Annotated SNP/indel list for targeted regions (dbsnp, snp type, refseq genes, etc.)
  • SNPs/indels in VCF format
  • Genotyping-ready results delivered using VarSifter

Comprehensive Service Available
Variant Analysis PlatformVariantPro Targeted Sequencing Technology
Starting Sample MaterialYour genomic DNA sample
Sample RequirementAs low as 10ng
Species CoveredAny species for which reference genome exists
On-target Specificity> 97%
Coverage Uniformity> 97%
Full Data Analysis Included
Data Delivery Time4-6 Weeks

Also available – custom and standard panel capture kits

  • Target enrichment report, including capture specificity and completeness
  • Alignment to reference sequence
  • Quality metrics, including mapping statistics, hybridization and selection metrics, mapping stats, and basecall quality distributions
  • Annotated SNP/indel list for targeted regions (dbsnp, snp type, refseq genes, etc.)
  • SNPs/indels in VCF format
  • Genotyping-ready results delivered using VarSifter