LC Sciences offers a customized solution to high-throughput genome wide variant analysis. The VariantPro™ system is an innovative multiplex PCR based targeted sequencing method that accomplishes target selection, library preparation and molecular tagging in a simple one-step workflow. The method incorporates novel Relay-PCR™ and Omega Primer™ technologies to produce amplicons of high uniformity & specificity.
|Description||The assay kit contains all the reagents required for capture of your custom targets and library preparation for next-gen sequencing|
|Capture Method||The VariantPro™ system incorporates novel Relay-PCR™ and Omega Primer™ technologies to form a one-step multiplex PCR based workflow.|
|Number of Targets||Up to 20,000|
|Amplicon Length||Depends on sequencing platform|
|DNA Sample Source||Frozen tissue, whole blood, FFPE|
|Input DNA Required||10 ng per tube for human genomic DNA|
|Number of Tubes||1-2|
|Assay Time||4-6 hours|
|Hands-on Time||5 minutes|
|Sequencing Platform||Illumina, Ion Torrent|
|Multiplexing Capability||Up to 3024 samples in one lane|
|Coverage Uniformity||> 99% at 0.2 x mean|
VariantPro™ Capture Technology
VariantPro™ targeted sequencing system is based on a novel multiplex PCR technology that combines major innovations to facilitate simple operation and high performance in the preparation of targeted sequencing libraries.
- OmegaPrimer™ – a novel primer design that fundamentally improves primer specificity.
- RelayPCR™ – an elegant PCR method that combines multiple target capture and monoplex PCR amplification into a single reaction.
- Molecular Tags – used to identify individual PCR template molecules.