VariantPro™ Targeted Sequencing

LC Sciences offers a customized solution to high-throughput genome wide variant analysis. The VariantPro™ system is an innovative multiplex PCR based targeted sequencing method that accomplishes target selection, library preparation and molecular tagging in a simple one-step workflow.

  • Single tube, one step workflow
  • Only 5 minutes of hands-on time required
  • Compatible with both Illumina and Ion Torrent sequencing technologies
  • Sample types – genomic DNA, fresh frozen tissue, FFPE
  • Inputs as low as 10 ng

  • > 20,000 targets in a single experiment
  • > 3,000 error correcting barcodes available
  • On-target specificity > 97%
  • Coverage uniformity > 97%
  • Molecular tagging – PCR and sequencing induced biases and errors are detected and then removed

The VariantPro™ targeted sequencing system offers cost-effective, minimal hands-on, ultra-high resolution sequence analysis, that enables accurate detection of rare variants. It utilizes novel and patent pending Relay-PCR™ and Omega-Primer™ technologies.

VariantPro™ Custom Amplicon Panels
Discover germline or somatic mutations, rare transcripts and transcript variants, such as single nucleotide mutations (SNP). Detect low frequency alleles . High throughput genotyping…

VariantPro™ Standard Content Panels
Designed for coverage of known coding regions, the VariantPro™ Standard Panels offer cost-effective, ultra-high resolution sequence analysis for revealing clinically relevant mutations…

VariantPro™  Sequencing Services
Our “Sample to Data” comprehensive service includes: primer designs, sample QC, sample preparation (target selection, sample bar-coding, molecular tagging, library amplification)…

VariantPro™ targeted sequencing system is based on a novel multiplex PCR technology that combines major innovations to facilitate simple operation and high performance in the preparation of targeted sequencing libraries. The innovations are:

  1. OmegaPrimer™ – a novel primer design that fundamentally improves primer specificity and yet is capable of sustaining certain local template variations such as SNPs.
  2. RelayPCR™ – an elegant PCR method that combines multiple target capture and monoplex PCR amplification into a single reaction and therefore simplifies targeted sequencing library preparation.
  3. Molecular Tags – used to identify individual PCR template molecules. Relay-PCR™ and Omega Primer™ in combination provides a perfect vehicle to carry the molecular tags into amplicons.