OligoMix®

OligoMix® is a pooled mixture of oligonucleotide sequences used in high-throughput applications. This oligo mixture has a broad array of uses in several genomics applications such as CRISPR guide RNAs or FISH probes.

We synthesize thousands of oligonucleotide sequences at once in parallel and then cleave the oligos releasing them into solution in a single microtube. Synthesis occurs via standard DMT chemistry assuring efficient stepwise yield and high quality final product. The product is delivered as a pool in a single microtube – ready to use for your experiment.

Product Description mix of DNA oligonucleotide sequences
Number of Oligos thousands of sequences or more per tube
Oligo Form single stranded, desalted and ready for reactions
Length up to 150mers
5′ or 3′ terminus Modifications phosphate, fluorescent dyes, biotin, linkers, and others (inquire)
Internal Modifications modified DNA or RNA bases
Yield *tens of attomoles per sequence and a total of sub-fmols per OligoMix® tube
Delivery 14 days

*One fmole per sequence is the calculated amount of starting material for oligo synthesis. As with all standard oligonucleotide synthesis, the resulting final material will be of less quantity depending on the sequence length, composition, and other factors.

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Library Construction
In CRISPR-Cas9 mutation screens, guide RNAs targeting tens of thousands of sites within genes are cloned into viral vectors and delivered as a pool into target cells along with Cas9. By identifying guide RNAs that are enriched or depleted in cells…

Synthetic Biology
One limitation for gene synthesis is the cost of making the building blocks (oligonucleotides) that are assembled together to make genes. Multiplex, parallel DNA construction on a large scale requires pools of large numbers of short synthetic oligos.…

Sequence Capture
Several academic and commercial groups have developed a variety of capture methods for enriching or selectively amplifying subsets of the genome for targeted sequencing. The key performance parameters of these methods are…

FISH Applications
Developing a FISH assay requires the use of oligonucleotide probe sets, like oligopaint probes, which are fluorescently labeled, single-stranded DNA oligonucleotides that can be used to visualize genomic regions ranging in size from tens of kilobases to many.…

  1. Economical – At less than 0.8¢ per base, OligoMix is about 20 times more cost and time efficient than conventional oligos. Delivered in a single microtube, it enables inexpensive genome-scale experiments.
  2. Customizable – Customers can specify each oligonucleotide sequence (lengths up to 150-mers). We can synthesize oligonucleotides in OligoMix containing labels, such as terminus phosphate, amino and thiol with linkers, biotin, FAM or other dyes.
  3. Reliable – Innovative microfluidic array platform ensures high quality synthesis. Multiple QC steps are implemented at various stages of OligoMix manufacturing. OligoMix is subjected to both hybridization and qRT-PCR assays to assess final quality.
  4. Simple & Fast – Download our excel spreadsheet order form, paste in your sequences and email back to us. Product can be delivered in 1-2 weeks.

Microfluidic Array Platform—in situ Synthesis

OligoMix® achieves high synthesis purity because it is produced via an advanced microarray synthesis technology (µParaflo®) that integrates a photo-generated acid (PGA) chemistry, digital photolithography (DLP), and advanced microfluidics to enable high throughput parallel synthesis of custom DNA microarrays. The PGA chemistry enables the use of standard oligo building blocks, and eliminates the need for any specially modified nucleotides which may exhibit lower coupling efficiency. DLP technology enables programmable synthesis of custom sequences and the µParaflo® microfluidic device contains the synthesis reactions each within a picoliter-scale reaction chamber, producing more uniform synthesis than reactions performed on the open surface of a slide.

Biomarker

  • Conventional Chemicals
  • Established Synthesis Processes

  • Efficient Stepwise Yield
  • Quality Final Product

Synthesis Technology References

  • Gao X, Yu PY, LeProust E, Sonigo L, Pellois JP, Zhang H. (1998) Oligonucleotide synthesis using solution photogenerated acids. Journal of the American Chemical Society 120, 12698-12699 [abstract].
  • Srivannavit O, Gulari M, Gulari E, LeProust E, Pellois JP, Gao X, Zhou X. (2004) Design and fabrication of microwell array chips for a solution-based, photogenerated acid-catalyzed parallel oligonucleotide DNA synthesis. Sensors and Actuators A. 116, 150-160 [abstract].
  • Zhou X, Cai S, Hong A, Yu P, Sheng N, Srivannavit O, Yong Q, Muranjan S, Rouillard JM, Xia Y, Zhang X, Xiang Q, Ganesh R, Zhu Q, Makejko A, Gulari E, Gao X. (2004) Microfluidic picoarray synthesis of oligodeoxynucleotides and simultaneously assembling of multiple DNA sequences. Nucleic Acids Research 32, 5409-5417 [abstract].
  • Tian J, Gong H, Sheng N, Zhou X, Gulari E, Gao X, Church G. (2004) Accurate multiplex gene synthesis from programmable DNA chips. Nature 432, 1050-1054 [abstract].