Identification, classification and quantitation of microbes and bacteria within complex biological mixtures
Microbiome analysis is performed via 16S rRNA gene sequencing. 16S sequencing is commonly used for identification, classification and quantitation of microbes within complex biological mixture such as human gut microbiome samples1). The 16S rRNA gene is a highly conserved component of the transcriptional machinery of all DNA-based life forms2 and thus is highly suited as a target gene for sequencing DNA in samples containing up to thousands of different species.
How It Works:
Universal PCR primers can be designed to target the conserved regions of 16S making it possible to amplify the gene in a wide range of different microorganisms from a single sample. Conveniently, the 16S rRNA gene consists of both conserved and variable regions. While the conserved region makes universal amplification possible, sequencing the variable regions allows discrimination between specific different microorganisms such as bacteria, archaea and microbial eukarya.
Compared to traditional identification methods such as cloning and/or culturing, 16S rDNA sequencing of bacteria is a faster and more accurate method.
A comprehensive upgrade to dual-zone (V3 + V4) testing, enables longer sequence and more accurate analysis of colonies.
Requires much lower sequencing depth compared to metagenomic sequencing enabling multiplexing of many samples per sequencing run.
Efficient PCR amplification enables identification of extremely low abundance bacteria.