The study of basic microRNA (miRNA) function has been performed mainly through collection of tissues by invasive methods. However, in a clinical setting, more convenient and non-invasive methods are required, such as collection of peripheral blood or other bodily fluids.

For those of you currently collecting sample from patients or working on development of a clinical test, we have put together some recommendations for sample collection and preservation and for RNA extraction methods from bodily fluids.

Recently, it has been demonstrated that miRNAs are present in circulating blood plasma, both free circulating and within exosomes, as well as within other biofluids such as cerebral spinal fluid, urine and even breast milk. Circulating miRNAs in biofluids represent a potential new approach for diagnostic screening. Indeed, characteristic changes in the serum or plasma miRNA profiles of several cancers or other conditions have identified unique signatures that could be exploited as novel biomarkers in the clinic.

Related Service

miRNA/Small RNA Sequencing Service – miRNA sequencing is a new method and powerful tool to identify and quantitatively decode the entire population of microRNAs in your sample. LC Sciences now provides a comprehensive microRNA sequencing service[Learn more…]

Related Service

miRNA Microarray Service – LC Sciences provides a microRNA (miRNA) expression profiling service using microarrays based on our in-house developed µParaflo® technology platform. We have standard arrays for all mature miRNAs of all species available in the latest version of the miRBase database (Release 21, July 2014). Our service is comprehensive and includes sample labeling, array hybridization, image data processing and in-depth data analysis. Two-three weeks after receiving your total RNA samples, we’ll send you both the raw and fully analyzed data. [Learn more…]

Suppressing regulation of pancreatic cancer Identifying specific cancer associated proteins and microRNAs contained within exosomes