Using LC Sciences’ miRNA microarray service, researchers examined differential miRNA expression and determined that miR-208a and miR-199a may have contributed to THRβ-mediated cardiac hypertrophy, as indicated by the absence of T3-regulated ventricular expression in TRβΔ337T mice. Investigators conclude that THRβ plays an important role in the regulation of specific mRNA and miRNA in T3-induced cardiac hypertrophic growth and in the alteration of heart functions.
Mouse miRNA was analysed by LC Sciences (Houston, TX). Nine miRNAs were differentially expressed (P<0.05): four miRNAs were down-regulated, and five were up-regulated in TRβΔ337T mice compared with TRβWT. Three animals were evaluated per group.
miRNA Microarray Service – LC Sciences provides a microRNA (miRNA) expression profiling service using microarrays based on our in-house developed µParaflo® technology platform. We have standard arrays for all mature miRNAs of all species available in the latest version of the miRBase database (Release 21, July 2014). Our service is comprehensive and includes sample labeling, array hybridization, image data processing and in-depth data analysis. Two-three weeks after receiving your total RNA samples, we’ll send you both the raw and fully analyzed data. [Learn more…]
G.E. Império, I.P. Ramos, L.A. Santiago, G.F. Pereira, N.A. dos Santos Almeida, C.S. Fuziwara (2015) The Impact of a Non-Functional Thyroid Receptor Beta upon Triiodotironine-Induced Cardiac Hypertrophy in Mice Cell Physiol Biochem 7(2):477-90. [article]