Analysis of the exosomal content using miRNA microarray identified miR-1587 as a mediator of the exosomal effects on GSCs, in part via down-regulation of the tumor suppressive nuclear receptor co-repressor NCOR1. These results illuminate the tumor-supporting role for GA-hMSCs by identifying GA-hMSC-derived exosomes in the intercellular transfer of specific miRNA that enhance the aggressiveness of glioblastoma.
GA-hMSC–derived exosomes contain a unique miRNA profile.
A and B, miRNA profiles for GA-hMSC and GA-hMSC–derived exosomes, demonstrating a significant (P < 0.001) difference in miRNA content. A, Three hundred and twenty miRNAs were selected by identifying the top 200 miRNAs for each pair of GA-hMSC and GA-hMSC–derived exosomes according to the absolute fold change and collapsing them into nonduplicated miRNAs. B, The top 20 miRNAs and the 8 miRNAs that were highly expressed and highly enriched in exosomes were selected as in A and subjected to cluster analysis. C, Distribution of the average expression level for miRNA in exosomes four GA-HMSC lines, compared with the parental cell. Exosome-enriched miRNAs were both highly expressed (>5,000 hybridization intensity) and highly enriched (>3.0 SD) compared with exosome-depleted miRNA, which were both highly expressed (>5,000 hybridization intensity) and highly enriched (<−1.0 SD) in parental cells. D, Expression levels of predicted gene targets of the exosomal miRNA are significantly (P < 0.01) decreased in GSCs after treatment with GA-hMSC–derived exosomes. Exo, exosome.
miRNA Microarray Service – LC Sciences provides a microRNA (miRNA) expression profiling service using microarrays based on our in-house developed µParaflo® technology platform. We have standard arrays for all mature miRNAs of all species available in the latest version of the miRBase database (Release 21, July 2014). Our service is comprehensive and includes sample labeling, array hybridization, image data processing and in-depth data analysis. Two-three weeks after receiving your total RNA samples, we’ll send you both the raw and fully analyzed data. [Learn more…]
J. Figueroa, L. M. Phillips, T. Shahar, R. G. Verhaak F. K. Lang, et al. (2017) Exosomes from Glioma-Associated Mesenchymal Stem Cells Increase the Tumorigenicity of Glioma Stem-like Cells via Transfer of miR-1587 American Association for Cancer Research doi: 10.1158/0008-5472.CAN-16-2524 [abstract]