Nov

4

For Synthetic Biology Applications: Go Green with OligoMix®

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Why synthesize thousands of oligonucleotides individually?

Why perform thousands of individual reactions while handling thousands of tubes?

When you can

Synthesize one OligoMix®.
Perform thousands of parallel (multiplex) reactions in a single tube.
Save time and money.
Be kind to our environment.
 
For instance; for a small genome of 5Mbp, 250,000 oligos of 40mers are required.  To synthesize thousands of oligo sequences individually would be expensive, equipment intensive, impractical.

Making thousands of oligonucleotides consumes a huge amount of solvents and chemical reagents which is environmentally-unfriendly, costly, and time consuming.  The synthesis per se would need 60,000 liters of solvents and cost $1M.  It would take a gene synthesis company a year to complete.

DNA synthesis of individual oligonucleotides is a major operation requiring significant up-front investment in synthesis instrumentation.  Significant personnel are required for time consuming synthesis and handling processes.

Performing multiplex applications quickly and accurately using synthetic oligonucleotides in individual tubes or plates requires sophisticated robotic instrumentation that is maintenance intensive and requires highly technical personnel.

Imagine if one person increases experimental throughput by 100-fold, one would need 100-times more glass and plastic-ware and 100-times more material consumption.  If there is no change in the way we do things, our research budget and space requirements would grow by 100-fold or more! 

Consider OligoMix® as a green alternative.

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Sep

16

Putting Synthesis into Biology: A Viral View of Genetic Engineering through De Novo Gene and Genome Synthesis

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S1074552109X00049_cov150hThe rapid improvements in DNA synthesis technology hold the potential to revolutionize biosciences in the near future. Traditional genetic engineering methods are template dependent and make extensive but laborious use of site-directed mutagenesis to explore the impact of small variations on an existing sequence “theme.” De novo gene and genome synthesis frees the investigator from the restrictions of the pre-existing template and allows for the rational design of any conceivable new sequence theme.Viruses, being among the simplest replicating entities, have been at the forefront of the advancing biosciences since the dawn of molecular biology. Viral genomes, especially those of RNA viruses, are relatively short, often less than 10,000 bases long, making them amenable to whole genome synthesis with the currently available technology. For this reason viruses are once again poised to lead the way in the budding field of synthetic biology—for better or worse. (read more)