Feb

23

Peptide Arrays – Protocol

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11. Antimicrobial Peptide Arrays for Detection of Inactivated Biothreat Agents By: Chris R. Taitt1 , Stella H. North1, Nadezhda V. Kulagina1

Arrays of immobilized antimicrobial peptides are used to detect bacterial, viral, and rickettsial pathogens, including inactivated biothreat agents. These arrays differ from the many combinatorial peptide arrays described in the literature in that the peptides used here have naturally evolved to interact with and disrupt microbial membranes with high affinity but broad specificity. The interaction of these naturally occurring peptides with membranes of pathogens has been harnessed for the purpose of detection, with immobilized antimicrobial peptides acting as “capture” molecules in detection assays. Methods are presented for immobilizing the antimicrobial peptides in planar arrays, performing direct and sandwich assays, and detecting bound targets.

Affiliation(s): (1) US Naval Research Laboratory, Washington, DC, USA

Book Title: Peptide Microarrays: Methods and Protocols Series: Methods in Molecular Biology | Volume: 570 | Pub. Date: Aug-01-2009 | Page Range: 233-255 | DOI: 10.1007/978-1-60327-394-7_11

Subject: Protein Science

Key Words: Biothreat – detection, array – antimicrobial peptide

Sep

21

Peptide Microarrays Discussed in Recent GEN Article

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Peptide drug discovery is a huge endeavor—and a huge field. Researchers and tool/technology developers alike traveled to Seoul recently for BIT Life Sciences’ “PepCon” meeting, where advances and trends in peptide and protein research were shared. (read more)

Sep

18

Phosphoprotein Binding – Peptide Microarray

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Sep

1

PepArray Featured in Recent GEN Article

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Peptide Therapies Coming Into Their Own
Source: www.genengnews.com
Peptide drug discovery is a huge endeavor—and a huge field. Researchers and tool/technology developers alike traveled to Seoul recently for BIT Life Sciences’ “PepCon” meeting, where advances and trends in peptide and protein research were shared.