May

24

LC Sciences Presents a Poster at PEGS 2010

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Epitope Mapping on a Flexible High-Density Microfluidic Chip – Peparray™

Ailing Hong1, Qi Zhu2, Yulu Zhang3, Xiaochuan Zhou1,2, Christoph Eicken2, Xiaolian Gao3,

1Atactic Technologies, and 2LC Sciences, Houston, TX 77054, 3Dept of Biology and Biochemistry, University of Houston, Houston, TX 77004

Although a number of proteomics technologies are well-developed, the demand for reliable, sensitive, accurate, and comprehensive measurements of epitope binding remains unfulfilled. Through the use of overlapping peptides as epitopes on a custom synthesized addressable peptide microarray (PepArray™), we can systematically screen thousands of epitope sequences in a single experiment.  A proprietary microarray platform and advanced microfluidic technologies ensure quantitative measurements of binding events. This combination of high-throughput capacity with quantitative measurement enables us to quickly and efficiently identify high affinity and high specificity target binding compounds. We have a novel, in situ, on-microchip synthesis technology (mParaflo®) that allows us to perform in situ synthesis of high-density peptide arrays according to custom designs. Forty-one 96-well titer plate experiments (i.e. conventional experiments) can be accomplished simultaneously on one chip. We also apply this flexible peptide array technology for rapid and reliable quantitative measurements for phosphorylation and protein binding. Our ultimate goal is to provide new, powerful research and clinical study tools to address the critical needs in proteomic research, clinical diagnosis, and therapeutic treatment.

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Mar

18

Epitope Mapping by Printed Peptide Libraries

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Frank Breitling1 , Christopher Schirwitz2, Thomas Felgenhauer2, Ines Block2, Volker Stadler2 and Ralf Bischoff2

(1)  Karlsruhe Institute of Technology, Helmholtzplatz 1, 76344 Eggenstein-Leopoldshafen, Germany
(2)  AG Chipbasierte Peptidbibliotheken, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany

 

Affordable high-density peptide arrays are needed to routinely define the exact binding sites of antibodies. In terms of prize and density peptide arrays currently lag far behind oligonucleotide arrays that are available in densities exceeding 50.000 oligonucleotides per cm2. This is mainly due to the monomer-by-monomer repeated consecutive coupling of 20 different amino acids associated with the lithographic methods, which adds up to an excessive number of coupling cycles. The combinatorial synthesis of peptide arrays based on electrically charged solid amino acid particles circumvents this problem. A colour laser printer or a microchip consecutively address the different charged particles to a solid support, where a complete layer of solid amino acid particles is melted at once. This releases hitherto immobilized amino acids to couple all 20 different amino acids to the support in one single coupling reaction.

 

LC Sciences offers a comprehensive epitope mapping service for high throughput, high-resolution identification of epitopes and other protein-protein interactions.

Through the use of overlapping peptides as epitopes on a custom synthesized addressable peptide microarray (PepArray™), we can systematically screen thousands of sequences in a single experiment.  A proprietary microarray platform and advanced microfluidic technologies ensure quantitative measurements of binding events.

This combination of high-throughput capacity with quantitative measurement enables us to quickly and efficiently identify high affinity and high specificity target binding compounds.

Feb

23

Peptide Arrays – Protocol

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11. Antimicrobial Peptide Arrays for Detection of Inactivated Biothreat Agents By: Chris R. Taitt1 , Stella H. North1, Nadezhda V. Kulagina1

Arrays of immobilized antimicrobial peptides are used to detect bacterial, viral, and rickettsial pathogens, including inactivated biothreat agents. These arrays differ from the many combinatorial peptide arrays described in the literature in that the peptides used here have naturally evolved to interact with and disrupt microbial membranes with high affinity but broad specificity. The interaction of these naturally occurring peptides with membranes of pathogens has been harnessed for the purpose of detection, with immobilized antimicrobial peptides acting as “capture” molecules in detection assays. Methods are presented for immobilizing the antimicrobial peptides in planar arrays, performing direct and sandwich assays, and detecting bound targets.

Affiliation(s): (1) US Naval Research Laboratory, Washington, DC, USA

Book Title: Peptide Microarrays: Methods and Protocols Series: Methods in Molecular Biology | Volume: 570 | Pub. Date: Aug-01-2009 | Page Range: 233-255 | DOI: 10.1007/978-1-60327-394-7_11

Subject: Protein Science

Key Words: Biothreat – detection, array – antimicrobial peptide

Sep

1

PepArray Featured in Recent GEN Article

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Peptide Therapies Coming Into Their Own
Source: www.genengnews.com
Peptide drug discovery is a huge endeavor—and a huge field. Researchers and tool/technology developers alike traveled to Seoul recently for BIT Life Sciences’ “PepCon” meeting, where advances and trends in peptide and protein research were shared.