Jun
24
New Higher Density Array
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30K Custom Array Design - LC Sciences has completed development of a new microfluidic chip design containing 30K features. We are currently accepting early access orders on a case-by-case basis for custom arrays built on this design for both microRNA profiling and epitope mapping applications. All of our array designs are based on the patented µParaflo® custom microarray platform.
May
28
microRNA Study in Non-Model Species
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LC Sciences’ custom microRNA microarray service is based on the flexible µParaflo® microfluidic chip technology which enables us to produce custom synthesized microarrays when ordered. (vs. an off-the-shelf spotted array) Therefore, researchers are not limited to the species (and sequences) listed in miRBase. They can add any sequence of their design to the standard miRBase probe content.
Customizable features include – sequence design, varying chain lengths, chip layout, synthesis chemistry, and more. Each µParaflo® microfluidic chip has room for thousands of sequences of your design. Add sequences for various applications:
- Screen for new microRNAs by adding predicted mature microRNA sequences or perform sequence tiling along certain sequences sections.
- Combine microRNA sequences of different species to identify cross-species conservations or host-parasite interaction.
- Add controls of customer’s choice for the detection of customer-added spiking RNA sequences and use as customer-selected internal controls.
- Add probes for the detection of siRNAs and/or other small non-coding RNAs.
- Liu G, Fang Y, Zhang H, Li Y, Li X, Yu J, Wang X. (2010) Computational identification and microarray-based validation of microRNAs in Oryctolagus cuniculus. Mol Biol Rep [Epub ahead of print] [abstract]
- Legeai F, Rizk G, Walsh T, Edwards O, Gordon K, Lavenier D, Leterme N, Mereau A, Nicolas J, Tagu D, Jaubert-Possamai S. (2010) Bioinformatic prediction, deep sequencing of microRNAs and expression analysis during phenotypic plasticity in the pea aphid, Acyrthosiphon pisum. BMC Genomics 11(1), 281. [abstract]
- Gundersen-Rindal DE, Pedroni MJ. (2010) Larval stage Lymantria dispar microRNAs differentially expressed in response to parasitization by Glyptapanteles flavicoxis parasitoid. Arch Virol (5), 783-87. [abstract]
- Dongdong L, Yusheng Zheng, Li Wan, Xiaoming Zhu and Zhekui Wang. (2009) Differentially expressed microRNAs during solid endosperm development in coconut (Cocos nucifera L.). Scientia Horticulturae 122(4), 666-69. [abstract]
- Sehm T, Sachse C, Frenzel C, Echeverri K. (2009) miR-196 is an essential early-stage regulator of tail regeneration, upstream of key spinal cord patterning events. Dev Biol 334(2), 468-80. [abstract]
Mar
10
It is known that Rho-associated kinase (ROCK) signaling plays a fundamental role in regulating cell morphology, adhesion, and motility and that aberrant expression of ROCK is related to tumor metastases and poor clinical outcome. Researchers at Tufts University proposed that ROCK may enhance the metastatic propensity of breast cancer cells by promoting the c-Myc pathway, including transcription of c-Myc–regulated miRNAs (miR-17-92 cluster)1. They used LC Sciences microRNA microarray services to show a 2- to 6-fold increase in expression of the miR-17-92 cluster in two metastatic breast cancer cell lines compared with non metastatic cells. The miR-17-92 expression in the three cell lines was validated by endpoint and qRT-PCR. Additionally, they showed that an anti-miR can block the ROCK signaling pathway resulting in decreased breast cancer cell invasion/ migration and metastasis. Therefore, inhibition of ROCK-mediated signaling appears to be a promising and potentially specific approach to suppress breast cancer metastases.
Numerous miRNAs have been shown to act as positive and negative regulators of the phosphoinositide-3-kinase (PI3K)/Akt-signaling pathway. The miR-29 family and miR-126 negatively affect the pathway through repression of PI3K regulatory subunits and many miRNAs positively influence PI3K/Akt signaling by targeting phosphatase and tensin homolog (PTEN) for inhibition which negatively affect phosphoinositide-3-kinase (PI3K)/Akt signaling. Researchers at the University of Texas Southwestern Medical Center, Dallas made use of LC Sciences microRNA microarray analysis and found that miR-486 showed a dramatic increase in expression in myocardin-related transcription factor-A (MRTF-A)–transduced cells2. The induction of miR- 486 by MRTF-A was confirmed by Northern blot and real-time RT– PCR. PTEN is a strongly predicted target of miR-486 and they further demonstrated that inhibition of miR-486 expression enhances the expression of PTEN and dampens signaling through the PI3K/Akt-signaling pathway. These findings implicate miR- 486 as another potential modulator of PI3K/Akt signaling.
- Liu S, Goldstein RH, Scepansky EM, Rosenblatt M. (2009) Inhibition of rho-associated kinase signaling prevents breast cancer metastasis to human bone. Cancer Res 69(22), 8742-51. [abstract]
- Small EM, O’Rourke JR, Moresi V, Sutherland LB, McAnally J, Gerard RD, Richardson JA, Olson EN. Regulation of PI3-kinase/Akt signaling by muscle-enriched microRNA-486. Proc Natl Acad Sci U S A 107(9), 4218-23. [abstract]
Dec
15
LC Sciences’ microRNA microarrays cover all species for which sequence data are available in the miRBase Sequence Database and the Plant MicroRNA Database. Other suppliers are not covering miRs from the Plant MicroRNA database and their arrays covering the miRBase are somewhat out of date. Since miRBase is being continually updated, a flexible microarray platform is necessary to keep current with the latest miRBase version (14.0 released in September of 2009).
| Supplier | miRBase Version Coverage | Total miRBase Entries | miRBase Release Date |
| LC Sciences | 14.0 | 10883 | Sept 2009 |
| Agilent | 12.0 | 8619 | Sept 2008 |
| Affymetrix | 11.0 | 6396 | April 2008 |
| Exiqon | 11.0 | 6396 | April 2008 |
| Invitrogen | 9.0 | 4361 | Oct 2006 |
Dec
10
Featured Customer Publication – Expression Profile of MicroRNAs in Young Stroke Patients
Filed Under New Customer Publications | Leave a Comment
Researchers at the University of Malaya, Kuala Lumpur and the National University of Singapore demonstrate that peripheral blood miRNAs and their profiles can be developed as biomarkers in diagnosis and prognosis of cerebral ischaemic stroke. MicroRNA microarrays were utilized to detect dysregulated miRNAs even after several months from the onset of stroke in what is usually regarded as neurologically stable patients.
Tan KS, Armugam A, Sepramaniam S, Lim KY, Setyowati KD, Wang CW, Jeyaseelan K. (2009) Expression profile of MicroRNAs in young stroke patients. PLoS One 4(11), e7689. [article]
Dec
2
UNLOCKING THE SECRETS OF microRNA
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By Anne Harding
Microarrays are a vital tool in research on microRNAs, the tiny noncoding stretches of genetic material that regulate messenger RNA. They can’t be beat for reading the microRNA “fingerprints” unique to disease states. But keeping up with this super hot field is a challenge for commercial microarray makers, and competition is fierce. (read more)
Nov
11
Researchers at the LSU Neuroscience Center have discovered a role for miRNA-146a in the evasion of Herpes simplex virus type-1 (HSV-1) from the complement system (a major first-line host defense mechanism), and the activation of key elements of the arachidonic acid cascade known to contribute to Alzheimer-type neuropathological change.
MicroRNA microarray revealed that human primary neural cells infected with HSV-1 (17syn +) showed upregulation of miRNA-146a, a brain-enriched microRNA that is associated with proinflammatory signaling in stressed brain cells and Alzheimer’s disease.
Hill JM, Zhao Y, Clement C, Neumann DM, Lukiw WJ. (2009) HSV-1 infection of human brain cells induces miRNA-146a and Alzheimer-type inflammatory signaling. Neuroreport 20(16), 1500-505. [abstract]
Oct
30
MicroRNAs as Potential Biomarkers in Cancer and Heart Disease
Filed Under Technical Article | 2 Comments
Heart disease and cancer represent the number one and number two killer diseases in developed countries. Identifying biomarkers to detect these and other diseases at early stages remains an important research goal and researchers are scrambling for ways to identify contributing factors to the pathogenesis of diseased cells in the body. In recent years, research has turned to the study of microRNA (miRNA) as possible biomarkers due to their extensive role in biological processes and cell functionality in normal vs. diseased cells. Microarrays combined with quantitative real-time PCR (qRT-PCR) validation are proving to be valuable tools for miRNA expression profiling and are predicted to play a crucial role in biomarker discovery and detection. Read more
Oct
16
The most current probe content available delivering the most complete microRNA expression profile of your samples
miRBase Sequence Database Content – Release 14.0 – September 2009
The miRBase database provides a searchable online repository for published microRNA sequences and associated annotation.
miRBase 14 Probe content is available now at LC Sciences!
- 10883 entries representing hairpin precursor miRNAs
- 10581 mature miRNA products
- Verified miRNAs for 115 species
Plant MicroRNA Database (PMRD) Content – Release 1 – October 2009
The plant miRNA database (PMRD) integrates available plant miRNA data deposited in public databases, gleaned from the recent literature, and data generated by the database organizers. This database contains sequence information, secondary structure, target genes, expression profiles and a genome browser.
PMRD Probe content is available now at LC Sciences!
- 8433 miRNAs
- 121 plant species
- Includes model plants and major crops such as Arabidopsis, rice, wheat, soybean, maize, sorghum, barley, etc in this first edition of the database.
Customer Defined Content
These are not spotted arrays. Each microarray is custom synthesized when ordered. That means we can add custom content to any of our standard microRNA microarrays with no delay in data delivery. There is no charge for addition of up to 100 of your custom sequences. Furthermore, the entire contents of the arrays are completely customizable according to the customer’s design at a very affordable price. Customers may add up to 3,918 sequences of their own selection for various applications.
- Screen for new microRNAs by adding predicted mature microRNA sequences or performing tiling along a certain section of genome.
- Combine microRNA sequences of different species to identify cross-species conservations.
- Add controls of customer’s choice for the detection of customer-added spiking RNA sequences and use as customer-selected internal controls.
- Add probes for the detection of siRNAs and/or other small non-coding RNAs.
Oct
7
New Customer Publication – miRNA Target Identification
Filed Under New Customer Publications | Leave a Comment
Identifying the target mRNA against miRNA is essential to understanding cellular regulatory networks. However, due to the low complementarity between a miRNA and its target mRNAs in mammals, identification of mammalian miRNA targets has been a challenge and only a few have been reported to date. Researchers at the Genome Research Laboratories, Wako Pure Chemical Industries, Japan propose that the ability to isolate and profile both the mRNA and miRNA components of the RISC complex has the potential to aid in the identification of miRNA targets. They have developed a protocol for isolation of endogenous RISC-associated miRNAs and mRNAs by immunoprecipitation with antibodies specific for Ago2, an Argonaute family protein that is the central RISC component. They used LC Sciences microRNA microarray service to compare the profile the immunopurified RNA with that of total RNA. Microarray results suggested that Ago2-immunopurification could trap almost all expressed miRNA species. Subsequent cloning and analysis of both miRNAs and mRNAs correctly correlated a well-characterized miRNA with its target.
- Hayashida Y, Nishibu T, Inoue K, Kurokawa T. (2009) A useful approach to total analysis of RISC-associated RNA. BMC Res Notes 2,169. [abstract]
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LC Sciences in the News- microRNA News
- Scientists on path to new treatments for Parkinson's July 30, 2010
- Macro Roles of microRNAs in Rett Syndrome? - rettsyndrome.org July 29, 2010
- Rosetta Genomics Names Genekor S.A Exclusive Distributor for Three ... July 29, 2010
- Alimta Mesothelioma | Rosetta Genomics Names Genekor S.A Exclusive ... July 29, 2010
- Live Cell Monitoring of hiPSC Generation and Differentiation Using ... July 29, 2010
- Thinking In Genes » Cancer Cells Show Rewired, Fragmented microRNA ... July 29, 2010
