Jan

20

Seminar – MicroRNA Discovery & Profiling

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Presented by – Dr. Christoph Eicken & Dr. Qi Zhu, LC Sciences
12:00 pm Conference Room 2

as part of – Texas Medical Center BioResearch Product Faire

Houston, TX (Marriott Medical Center) – January 16, 2010

Oct

30

MicroRNAs as Potential Biomarkers in Cancer and Heart Disease

Filed Under Technical Article | 2 Comments

Heart disease and cancer represent the number one and number two killer diseases in developed countries. Identifying biomarkers to detect these and other diseases at early stages remains an important research goal and researchers are scrambling for ways to identify contributing factors to the pathogenesis of diseased cells in the body.  In recent years, research has turned to the study of microRNA (miRNA) as possible biomarkers due to their extensive role in biological processes and cell functionality in normal vs. diseased cells.  Microarrays combined with quantitative real-time PCR (qRT-PCR) validation are proving to be valuable tools for miRNA expression profiling and are predicted to play a crucial role in biomarker discovery and detection.  Read more

Oct

7

New Customer Publication – miRNA Target Identification

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1756-0500-2-169.fm

Identifying the target mRNA against miRNA is essential to understanding cellular regulatory networks. However, due to the low complementarity between a miRNA and its target mRNAs in mammals, identification of mammalian miRNA targets has been a challenge and only a few have been reported to date. Researchers at the Genome Research Laboratories, Wako Pure Chemical Industries, Japan propose that the ability to isolate and profile both the mRNA and miRNA components of the RISC complex has the potential to aid in the identification of miRNA targets. They have developed a protocol for isolation of endogenous RISC-associated miRNAs and mRNAs by immunoprecipitation with antibodies specific for Ago2, an Argonaute family protein that is the central RISC component. They used LC Sciences microRNA microarray service to compare the profile the immunopurified RNA with that of total RNA. Microarray results suggested that Ago2-immunopurification could trap almost all expressed miRNA species.  Subsequent cloning and analysis of both miRNAs and mRNAs correctly correlated a well-characterized miRNA with its target.

  1. Hayashida Y, Nishibu T, Inoue K, Kurokawa T. (2009) A useful approach to total analysis of RISC-associated RNA. BMC Res Notes 2,169.  [abstract]

Sep

22

Featured Publication

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An essential role of microRNAs in reactive oxygen species-mediat Researchers at the RNA and Cardiovascular Research Laboratory, New Jersey Medical School have studied microRNA in acute myocardial infarction by expression profiling of miRNA in rat hearts with microarray. Their results suggest that miRNAs may play important roles in the phathophysiology of AMI. Some miRNAs may be considered new therapeutic targets or even biomarkers for ischemic heart disease such as AMI. [abstract]