May

28

microRNA Study in Non-Model Species

Filed Under Information | Leave a Comment

LC Sciences’ custom microRNA microarray service is based on the flexible µParaflo® microfluidic chip technology which enables us to produce custom synthesized microarrays when ordered. (vs. an off-the-shelf spotted array)  Therefore, researchers are not limited to the species (and sequences) listed in miRBase.  They can add any sequence of their design to the standard miRBase probe content.

Customizable features include – sequence design, varying chain lengths, chip layout, synthesis chemistry, and more.   Each µParaflo® microfluidic chip has room for thousands of sequences of your design.  Add sequences for various applications:

  • Screen for new microRNAs by adding predicted mature microRNA sequences or perform sequence tiling along certain sequences sections.
  • Combine microRNA sequences of different species to identify cross-species conservations or host-parasite interaction.
  • Add controls of customer’s choice for the detection of customer-added spiking RNA sequences and use as customer-selected internal controls.
  • Add probes for the detection of siRNAs and/or other small non-coding RNAs.
  1. Liu G, Fang Y, Zhang H, Li Y, Li X, Yu J, Wang X. (2010) Computational identification and microarray-based validation of microRNAs in Oryctolagus cuniculus.  Mol Biol Rep [Epub ahead of print]  [abstract]
  2. Legeai F, Rizk G, Walsh T, Edwards O, Gordon K, Lavenier D, Leterme N, Mereau A, Nicolas J, Tagu D, Jaubert-Possamai S. (2010) Bioinformatic prediction, deep sequencing of microRNAs and expression analysis during phenotypic plasticity in the pea aphid, Acyrthosiphon pisum. BMC Genomics 11(1), 281.  [abstract]
  3. Gundersen-Rindal DE, Pedroni MJ. (2010) Larval stage Lymantria dispar microRNAs differentially expressed in response to parasitization by Glyptapanteles flavicoxis parasitoid. Arch Virol (5), 783-87.  [abstract]
  4. Dongdong L, Yusheng Zheng, Li Wan, Xiaoming Zhu and Zhekui Wang. (2009) Differentially expressed microRNAs during solid endosperm development in coconut (Cocos nucifera L.). Scientia Horticulturae 122(4), 666-69.  [abstract]
  5. Sehm T, Sachse C, Frenzel C, Echeverri K. (2009) miR-196 is an essential early-stage regulator of tail regeneration, upstream of key spinal cord patterning events. Dev Biol 334(2), 468-80.  [abstract]

Oct

16

The Most Current Probe Content Available

Filed Under Information | Leave a Comment

mirna_probe_contentThe most current probe content available delivering the most complete microRNA expression profile of your samples

miRBase Sequence Database Content – Release 14.0 – September 2009

The miRBase database provides a searchable online repository for published microRNA sequences and associated annotation. 

miRBase 14 Probe content is available now at LC Sciences!

  • 10883 entries representing hairpin precursor miRNAs
  • 10581 mature miRNA products
  • Verified miRNAs for 115 species

Plant MicroRNA Database (PMRD) Content – Release 1 – October 2009

The plant miRNA database (PMRD) integrates available plant miRNA data deposited in public databases, gleaned from the recent literature, and data generated by the database organizers. This database contains sequence information, secondary structure, target genes, expression profiles and a genome browser.

PMRD Probe content is available now at LC Sciences!

  • 8433 miRNAs
  • 121 plant species
  • Includes model plants and major crops such as Arabidopsis, rice, wheat, soybean, maize, sorghum, barley, etc in this first edition of the database.

Customer Defined Content

These are not spotted arrays.  Each microarray is custom synthesized when ordered.  That means we can add custom content to any of our standard microRNA microarrays with no delay in data delivery. There is no charge for addition of up to 100 of your custom sequences.  Furthermore, the entire contents of the arrays are completely customizable according to the customer’s design at a very affordable price.  Customers may add up to 3,918 sequences of their own selection for various applications.

  • Screen for new microRNAs by adding predicted mature microRNA sequences or performing tiling along a certain section of genome.
  • Combine microRNA sequences of different species to identify cross-species conservations.
  • Add controls of customer’s choice for the detection of customer-added spiking RNA sequences and use as customer-selected internal controls.
  • Add probes for the detection of siRNAs and/or other small non-coding RNAs.

Sep

24

µParaflo® Biochip Technology – Microfluidic Synthesis Platform

Filed Under Information | Leave a Comment

A platform technology that encompass a new class of microfluidic µParaflo® reaction devices, an advanced digital light synthesizer apparatus, and picoliter scale biochemical processes.  The functionalized µParaflo® chips are particularly suited for applications where small sample consumption, contamination-free, and performance-reproducibility are primary concerns. This technology enables the massively parallel synthesis of high quality DNA and RNA oligonucleotides as well as peptides and peptidomimetics in picoliter-scale reaction chambers. The only platform providing microarrays of oligonucleotide, peptide, and their analog sequences by in situ synthesis using conventional chemistry and a comprehensive list of off-the-shelf synthetic building blocks.

  • High Density – On Chip Parallel Synthesis
  • Customizable – DNA, RNA, Peptides, and Analogs
  • Versatile – Uses Conventional Chemistry