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Featured Customer Publication: Phloem small RNAs, nutrient stress responses, and systemic mobility

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Phloem small RNAs, nutrient stress responses, and systemic mobility

The authors used LC Sciences’ custom miRNA microarrays containing all known plant miRNAs and a set of unknown small (s) RNAs earlier cloned from Brassica phloem sap, to comprehensively analyze the phloem response to nutrient deficiency by removing sulfate, copper or iron, respectively, from the growth medium. They show that phloem sap contains a specific set of sRNAs that is distinct from leaves and roots, and that the phloem also responds specifically to stress. They further demonstrate that under nutrient starvation miR399 and miR395 can be translocated through graft unions from wild type scions to rootstocks of the miRNA processing hen1-1 mutant. In contrast, miR171 was not transported. Translocation of miR395 led to a down-regulation of one of its targets in rootstocks, suggesting that this transport is of functional relevance, and that miR395, in addition to the well characterized miR399, could potentially act as a long-distance information transmitter.

Buhtz A, Pieritz J, Springer F, Kehr J. (2010) Phloem small RNAs, nutrient stress responses, and systemic mobility. BMC Plant Biol 10(1), 64. [Article]

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