Small RNAs make up a large family of regulatory molecules in organisms of nearly all kingdoms of life. These include: miRNA, ncRNA, siRNA, snoRNA, piRNA, rasiRNA, and many more. Their functions are equally as diverse, including mRNA degradation, translational repression, hetero- chromatin formation, and DNA elimination as they play an important role in development and disease. Small RNA transcriptome sequencing is a new method and a powerful tool to identify and quantitatively decode the entire population of small RNAs and thus recover the families of regulatory RNAs in different organisms and cellular systems.
Small RNA Sequencing
- Profile small RNA in any species – there is no need for probes based on prior sequence or secondary structure information.
- Profiling is transcriptome-wide – investigate all small RNAs, of any size, known and unknown, in your sample.
- Achieve digital transcript expression analysis – Accurate quantitation of expression levels over 5 orders of magnitude of transcript abundance.
- Detect rare transcripts and transcript variants, such as single nucleotide mutations (SNP).
Our Comprehensive Service
LC Sciences provides a small RNA sequencing service using the Illumina high-throughput sequencing technology which enables comprehensive coverage, highly sensitive and specific discovery and profiling of all forms of small RNAs in your sample.
This service is comprehensive – from sample to data, providing advanced technology and years of experience in efficient discovery and profiling of small RNAs.
Our “Total RNA to Data” comprehensive service includes: sample QC, sample preparation (small RNA selection, library preparation, single molecule clonal amplification), high-throughput sequencing, bioinformatics analysis, and customer data report.
Down-stream profiling of identified (and other customer defined target small RNAs) is also available by custom microarray analysis service.
Data Analysis / Results
Standard Data Analysis
- Illumina base-calling and analysis
- LC Sciences analysis and quality filtering. Processed data is reduced to mappable reads.
- Customer data report – includes a list of unique sequences and their copy numbers
Advanced Analysis
- Custom construction of reference database(s) – miRBase, genome, etc and mapping of all quality reads
- Alignment, classification, & functional annotation of all mapped reads
- Prediction of possible novel miRs / small RNAs
- Biostatistical analysis – expression analysis, multi-parameter data analysis, length distribution, transcript copy number comparisons, etc
- Customer data report – includes a summary of methods and all statistic analysis
Custom Project Level Analysis
Custom project level service by LC Sciences’ small RNA experts providing integrated sequencing and custom microarray assays to serve the various needs of researchers from the research and biomedical fields. May include: differential expression and clustering analysis, pathway analysis (KEGG), GO term report, biomarker analysis, target prediction. Please contact us to learn how we can help get you the results you need to keep your research moving ahead.
Questions?
