Small RNAs make up a large family of regulatory molecules in organisms of nearly all kingdoms of life. These include: miRNA, ncRNA, siRNA, snoRNA, piRNA, rasiRNA, and many more. Their functions are equally as diverse, including mRNA degradation, translational repression, hetero- chromatin formation, and DNA elimination as they play an important role in development and disease. Small RNA transcriptome sequencing is a new method and a powerful tool to identify and quantitatively decode the entire population of small RNAs and thus recover the families of regulatory RNAs in different organisms and cellular systems.
- Profile small RNA in any species – there is no need for probes based on prior sequence or secondary structure information.
- Profiling is transcriptome-wide – investigate all small RNAs, of any size, known and unknown, in your sample.
- Achieve digital transcript expression analysis – Accurate quantitation of expression levels over 5 orders of magnitude of transcript abundance.
- Detect rare transcripts and transcript variants, such as single nucleotide mutations (SNP).
LC Sciences provides a small RNA discovery service using the Illumina high-throughput sequencing technology which enables comprehensive coverage, highly sensitive and specific discovery and profiling of all forms of small RNAs in your sample.
This service is comprehensive – from sample to data, providing advanced technology and years of experience in efficient discovery and profiling of small RNAs.
Our “Total RNA to Data” comprehensive service includes: sample QC, sample preparation (small RNA selection, library preparation, single molecule clonal amplification), high-throughput sequencing, bioinformatics analysis, and customer data report.
Optional target-specific selection service is available to enrich your sample(s) for more focused sequencing to achieve deeper coverage.
Custom microarrays have been shown to be a effective experimental approach to discovery of novel ncRNAs as well as a method to experimentally test computationally predicted ncRNAs. LC Sciences provides a small non-coding RNA discovery service using innovative µParaflo® technology and proprietary probe design, which enable highly sensitive and specific direct detection of small RNAs in your sample. This service is comprehensive; from array design to sample to data, allowing the most efficient novel discovery of small regulatory/functional RNA embedded within non-coding RNAs (ncRNAs) or genomic RNAs.
Our “Total RNA to Data” comprehensive service includes: Sample QC, Sample Preparation & labeling, small RNA Detection (hybridization to a µParaflo® microfluidics chip), Array Scan and Data Extraction, and Full Data Analysis.
In about 2-3 weeks you will receive: original and processed scan images, array layout and a list of sequences, raw and processed data, a list of differentially expressed transcripts (dual sample arrays only), and a summary of the results.