Small RNAs make up a large family of regulatory molecules in organisms of nearly all kingdoms of life. These include: miRNA, ncRNA, siRNA, snoRNA, piRNA, rasiRNA, and many more. Their functions are equally as diverse, including mRNA degradation, translational repression, hetero- chromatin formation, and DNA elimination as they play an important role in development and disease. Small RNA transcriptome sequencing is a new method and a powerful tool to identify and quantitatively decode the entire population of small RNAs and thus recover the families of regulatory RNAs in different organisms and cellular systems.
Small RNA Discovery Sequencing Service
- Profile small RNA in any species – there is no need for probes based on prior sequence or secondary structure information.
- Profiling is transcriptome-wide – investigate all small RNAs, of any size, known and unknown, in your sample.
- Achieve digital transcript expression analysis – Accurate quantitation of expression levels over 5 orders of magnitude of transcript abundance.
- Detect rare transcripts and transcript variants, such as single nucleotide mutations (SNP).
LC Sciences provides a small RNA discovery service using the Illumina high-throughput sequencing technology which enables comprehensive coverage, highly sensitive and specific discovery and profiling of all forms of small RNAs in your sample.
This service is comprehensive – from sample to data, providing advanced technology and years of experience in efficient discovery and profiling of small RNAs.
Our “Total RNA to Data” comprehensive service includes: sample QC, sample preparation (small RNA selection, library preparation, single molecule clonal amplification), high-throughput sequencing, bioinformatics analysis, and customer data report.
Optional target-specific selection service is available to enrich your sample(s) for more focused sequencing to achieve deeper coverage.
Small RNA Discovery Microarray Service
Custom microarrays have been shown to be a effective experimental approach to discovery of novel ncRNAs as well as a method to experimentally test computationally predicted ncRNAs. LC Sciences provides a small non-coding RNA discovery service using innovative µParaflo® technology and proprietary probe design, which enable highly sensitive and specific direct detection of small RNAs in your sample. This service is comprehensive; from array design to sample to data, allowing the most efficient novel discovery of small regulatory/functional RNA embedded within non-coding RNAs (ncRNAs) or genomic RNAs.
Our “Total RNA to Data” comprehensive service includes: Sample QC, Sample Preparation & labeling, small RNA Detection (hybridization to a µParaflo® microfluidics chip), Array Scan and Data Extraction, and Full Data Analysis.
In about 2-3 weeks you will receive: original and processed scan images, array layout and a list of sequences, raw and processed data, a list of differentially expressed transcripts (dual sample arrays only), and a summary of the results.
