LC Sciences provides a genome-wide microRNA (miRNA) expression profiling service using µParaflo® technology and proprietary probe design, which enable highly sensitive and specific direct detection of miRNAs. We have standard arrays for mature miRNA of all species available in the latest version of the miRBase database (Release 18). Our service is comprehensive and includes sample preparation of your total RNA sample, single or dual color labeling, hybridization, image data processing and in-depth data analysis. Two-three weeks after receiving your total RNA samples, we send you the analyzed data, representative and original images of the array, and raw data. We offer additional products and services including arrays containing miRNA*, walk/tiling arrays, custom arrays (first 100 probes free of charge), and in-depth analysis for multiple data sets. The in-depth data analysis may include multi-array normalization, t-test, ANOVA, False Discovery Rate calculator, and/or clustering analysis.
Special Announcement
miRBase Version 18 Content Now Available!
November 3rd , 2011 – The miRBase sequence database was just updated to version 18. Release 18 of the database contains 18226 entries representing hairpin precursor miRNAs, expressing 21643 mature miRNA products, in 168 species. As compared to miRBase version 17 a total of 1488 new hairpin sequences and 1929 novel mature products have been added.
This probe content is immediately available on all of our standard or custom microRNA microarrays.
Flexible content means LC Sciences always delivers the most comprehensive profile of microRNA expression in your sample.
Featuring µParaflo® Biochip Technology
A high performance microfluidic custom microarray platform.
- 100% miRBASE VERSION 18 CONTENT
- COMPLETE CONTENT FLEXIBILITY (All Species)
- OPTIMIZED RNA HYBRIDIZATION PROBES
- EXPERIENCED SERVICE PROVIDER HANDLING VARIOUS RESEARCH & CLINICAL SAMPLE TYPES
(Including FFPE and Blood/Plasma Samples) - COST EFFECTIVE 1-STOP SOLUTION
| Comprehensive Service Available | √ |
| Microarray Platform | µParaflo®Microfluidic Biochip Technology |
| Dual Color Capable | √ |
| Starting Sample Material | >Total RNA, miRNA, Blood,Cell Pellet, Tissue, FFPE |
| Sample Requirement | 5 µg Total RNA – If you are limited in sample amount, please give us a call. We can accommodate smaller sample sizes. |
| Probe Content | miRBase 18 (Nov 2011) |
| Species Covered | All species for which data exists (miRBase, etc.) |
| Probes Tm Equalized | √ |
| Add Custom Sequences | √ |
| Detection Limit | < 10 attomole |
| Dynamic Range | >3.5 logs |
| Full Data Analysis Included | √ |
| Data Delivery Time | 2-3 Weeks |
| Number of Customer Publications to Date | >250 |
Latest Product News
Nov 3, 2011 - The miRBase sequence database was just updated to version 18. Release 18 of the database contains 18226 entries representing hairpin precursor miRNAs, expressing 21643 mature miRNA products, in 168 species. As compared to miRBase version 17 a total of 1488 new hairpin sequences and 1929 novel mature products have been added. (read more…)
April 26, 2011 - The miRBase sequence database was just updated to version 17. 1626 new hairpin sequences and 2406 novel mature miR and miR* products have been added. This is a major update with large additions to well characterized species such as human and mouse plus the addition of 11 new species. (read more…)
Sept 9th, 2010 – The miRBase sequence database was just updated to version 16. 1068 new hairpin sequences and 1788 novel mature miR and miR* products have been added. This is a major update with large additions to well characterized species such as human, mouse, rat. (read more…)
April 26th, 2010 - miRBase 15 Content Now Available -The miRBase sequence database was just updated to version 15. This content is immediately available on all our standard and custom miRNA microarrays. Version 15 represents a 48% increase in the number of miRNA sequences over version 14. (read more…)
March 19th, 2010 – Introducing Seq-ArraySM This unique combination of the latest deep sequencing technology, advanced bioinformatics, and our innovative µParaflo® custom microarray platform leverages all these technologies to form a comprehensive service package tailored to your specific research needs. (read more…)
Dec 11th, 2009 – LC Sciences’ microRNA Microarray customers publish in Science Magazine- This groundbreaking work identified miR-206 as a modifier of Amyotrophic lateral sclerosis (ALS) pathogenesis and provides a new perspective on the mechanisms underlying this neurodegenerative disease. (read more…)
Sept 15, 2009 – With the addition of 1580 new mature miR and miR* sequences, the microRNA Sequence Database (miRBase) announced a significant update to the sequence database which has now topped 10,000 entries. (read more…)
March 9, 2009 – With the addition of 1004 new mature miRNA sequences, the Sanger microRNA Sequence Database (miRBase) announced a significant update to the miRBase sequence database. This latest release is miRBase version 13.0 and features a total of 9169 mature miRNA sequences. (read more…)
Overview of the Service
Microfluidics Microarray Platform
The µParaflo® technology integrates high throughput parallel synthesis, microfluidics, and digital photolithography to create custom arrays of DNA, RNA, or peptides. Our advanced microarray platform delivers more reliable results than are possible with traditional spotted arrays.
Optimized RNA Hybridization Probes
LC Sciences’ Optimized RNA Hybridization Probes are designed with normalized Tms to ensure uniform hybridization affinity to their microRNA targets under high stringency hybridization conditions enhancing both the sensitivity and specificity of the probes.
The Most Current Content
LC Sciences offers the only microRNA microarray currently available with version 18 probe content. Not 90% or 95% coverage, but 100% of experimentally verified microRNA sequences are represented on all of our arrays.
Complete Content Flexibility
All of LC Sciences’ microarrays are made to order and synthesized to each customer’s particular specifications. Validate predictions, add custom controls, or create totally custom tiling arrays for discovery of novel small-RNAs. The choice is up to you!
No Limitation on Use of Data
Unlike with proprietary probe content, we hold no claims on your data. You have full access and control over the results generated from your experiments.
The Standard for Array Data Quality
The µParaflo® technology enables on-chip synthesis ensuring high probe quality and tight process control. The data obtained by these microarrays is highly reliable and reproducible as validated by qPCR and Northern Blot.
In Depth Data Analysis
LC Sciences’ in-depth data analysis includes all t-Tests, ANOVA and heat maps to get you the information you need. Complex multi-array experiments are no problem for our experienced technical team. We can easily compare samples from different chips or even new experimental data to data from previous experiments.
The Most Customer Publications
LC Sciences’ customers have published over 250 papers to date; have confirmed that microRNAs play an important role in a wide array of biological functions.
