microRNA Sequencing Service

Small RNA Sequencing    RSQ-1035s     $1,100/lane

• High-throughput sequencing (Illumina sequencing by synthesis technology)
• Single lane 35 cycle run
• up to 30M raw sequencing reads per run
• Raw sequencing data report – either FASTA-A or FASTA-Q files with base-calling quality scores

Small RNA Library Preparation    MRS-1002     $400/sample

• Sample QC
• Small RNA selection
• Library preparation
• Single molecule clonal amplification

Standard Data Filtering Service    MRS-1006     Included

• Read quality analysis
• Filtering of sequencing data
• Data reduce to mappable reads
• Customer data report – includes LC Sciences analysis and quality filtering data and a list of unique sequences and their copy numbers

In Depth Data Analysis Service    MRS-1007     (Inquire)

• Map to Reference Sequence
• Includes custom construction of reference database(s) for mapping – miRBase, genome, etc
• Sequence data can be aligned to any publicly available small RNA databases for annotation of known small RNA
• Unknown small RNAs can be aligned with reference genomes
• Additional Bioinformatics Analysis
• Classification, & functional annotation of all mapped reads
• Prediction of possible novel miRs
• Biostatistical analysis – expression analysis, multi-parameter data analysis, length distribution, transcript copy number comparisons, etc
• Customer data report – includes a summary of methods and all statistic analysis

Also Available – Sample barcoding – The Illumina sequencing platform supports barcoding of samples for indexing to reduce number of sequencing runs required.  Additional sample preparation charges will apply.

Also Available – Down-stream profiling of identified (and other customer defined target microRNAs) is also available by custom microarray analysis service.

Seq-Array^SM is a combination of technologies, deep sequencing and custom microarray, that is particularly suited for focused studies consisting of large sample numbers. Following the exploratory sequencing, which defines the comprehensive set of sequences, microarray probe sequences are designed and efficient profiling on custom arrays is performed.

SeqArray™ Design Service    SAS-1001    (Inquire)

• Design of a custom uParaflo® microfluidic array
• Includes probe ID reference to known database (mirbase, genome, etc)
• Contains additional sequence variants derived from sequencing results
• Probe design takes consideration of sequencing counts
• Requires one of the following :