LC Sciences provides a comprehensive Gene Expression Analysis Service featuring both the Affymetrix and Agilent microarray platforms.
Both platforms have shown excellent performance and are proven as reliable methods for gene expression profiling. Additionally, both platforms offer a wide range of arrays.
Our service is comprehensive and includes target preparation of your total RNA sample, single or dual color labeling, hybridization, image data processing and in-depth data analysis. Two-three weeks after receiving your total RNA samples, we send you original images of the array, raw data and fully analyzed data.
In-depth data analysis of multiple sample group experiments may include:
- Multi-array normalization
- t-tests
- ANOVA
- Clustering analysis
- Heat maps
- GO enrichment
| Comprehensive Service Available | √ |
| Microarray Platforms Available | Affymetrix – Genechip® Technology Agilent – SurePrint G3 Expression Microarrays |
| Starting Sample Material | Total RNA, mRNA, Blood, Cell Pellet, Tissue, FFPE |
| Sample QC | Agilent 2100 Bioanalyzer RNA LabChip Nanodrop ND-1000 |
| Low input total RNA protocol available | √ |
| Species Covered | All Species for which Affy or Agilent Arrays Exist Inquire about custom arrays |
| Advanced Data Analysis Included | √ |
| Data Delivery Time | 2-3 weeks |
Advanced Data Analysis Deliverables:
Affymetrix Genechip Platform
We use such Affymetrix software systems as MicroArray Suite, MicroDB and Data Mining Tool to provide the data analysis service.
- Raw chip image in jpg format
- Raw array data including original .DAT,.EXP and .CEL files
- Analysis results for each array in Excel format including the Signal, Detection and Detection p-value information of all sample genes
- QC report for each array that and general information about hybridization of all sample arrays
- Computation of a global normalized expression metric using Robust Multiarray Average (RMA) of the hybridization intensity data from all chips in the experimental set
- Differential gene expression analysis of all samples (Note – number of analyses performed can be no more than the number of chips in service)
- Report in Excel including information on change p-value, increase or decrease
- Scatter plot of different samples which directly reflects the general difference and distribution of signal strength of all samples.
- Additional clustering analysis, heat maps, and GO enrichment as requested
Agilent Gene Expression Microarrays Platform
We use such Agilent software systems as GeneSpring software and/or Agilent Genomic Workbench to provide the data analysis service.
- Raw chip image in jpg format
- QC report for each sample
- QC report for each array that and general information about hybridization of all sample arrays
- Computation of a global normalized expression of the hybridization intensity data from all chips in the experimental set
- Differential gene expression analysis of all samples (Note – number of analyses performed can be no more than the number of chips in service)
- Report in Excel including information on change p-value, increase or decrease
- Scatter plot of different samples which directly reflects the general difference and distribution of signal strength of all samples
- Cluster analysis heat maps
- Gene Ontology (GO), Pathway and Statistical Enrichment analysis and related graphs/charts
- Additional advanced analysis as requested
