LC Sciences now offers a comprehensive epitope mapping service for high throughput, high-resolution identification of epitopes and other protein-protein interactions.
Through the use of overlapping peptides as epitopes on a custom synthesized addressable peptide microarray (PepArray™), we can systematically screen thousands of sequences in a single experiment. A proprietary microarray platform and advanced microfluidic technologies ensure quantitative measurements of binding events.
This combination of high-throughput capacity with quantitative measurement enables us to quickly and efficiently identify high affinity and high specificity target binding compounds.
Epitope mapping assays are applicable for immunological studies, vaccine development, and biosensor development.
- Map immunodominant region in an antigen
- Develop epitope-based vaccines
- Describe antibody binding in detail for regulatory or patent filings
- Determine the mechanism of a biological process
This is a comprehensive epitope mapping service. Just send us your protein/antibody sample and we’ll perform all the necessary functions from sample assay to data analysis. Our Epitope Mapping comprehensive service includes: assistance with your sequence designs, synthesis of a custom epitope mapping – peptide microarray, all on-chip binding reactions, detection of the bound antibody using standard immunological detection techniques, array image scan, and data analysis. We offer additional services for in-depth binding data analysis and curve analysis of quantitative measurements.
Epitope mapping on a peptide microarray offers the opportunity to study thousands (up to tens of thousands of peptides/chip) of specific binding sequences in a single experiment. One experiment using our 4K epitope mapping – peptide microarray is equivalent to 41 experiments using a 96-well plate. A 30K microarray format is also available.
Peptides are synthesized on-chip, not pre-synthesized and spotted. There is no up-front cost to pre-synthesize a peptide library. We can synthesize a completely custom one-of-a-kind peptide microarray specifically for your experiment delivering results that cannot be achieved with an off-the-shelf assay. With the ability to program new sequence designs on the fly, you can quickly revise microarray design and content to keep experiments moving forward based on previous results.
Microfluidic epitope mapping – peptide microarrays using our PepArray™ technology produce more uniform spots and enhanced binding kinetics to deliver a more reliable reading of the assay signals. Well designed positive and negative controls as well as multiple replicates of each peptide sequence contribute to data confidence.