Our genomics products and services include the use of custom synthesized microarrays based on the µParaflo® on-chip synthesis technology which enables the total customization of content on each individual microarray to suit your needs. In addition, the flexibility of µParaflo® enables the incorporation of modifications within the probes, which significantly expands the range of applications for these microarrays. Listed below are publications illustrating the application of custom microarrays containing non-standard probes.
Protein-Oligonucleotide Binding (Transcription Factor) Microarrays
Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17225-30.
Department of Biochemistry & Molecular Biology, University of Texas Medical School, Houston, TX 77030.
Transcription enhancer factor 1 is essential for cardiac, skeletal, and smooth muscle development and uses its N-terminal TEA domain (TEAD) to bind M-CAT elements. Here, we present the first structure of TEAD and show that it is a three-helix bundle with a homeodomain fold. Structural data reveal how TEAD binds DNA. Using structure-function correlations, we find that the L1 loop is essential for cooperative loading of TEAD molecules on to tandemly duplicated M-CAT sites. Furthermore, using a microarray chip-based assay, we establish that known binding sites of the full-length protein are only a subset of DNA elements recognized by TEAD. Our results provide a model for understanding the regulation of genome-wide gene expression during development by TEA/ATTS family of transcription factors.