Our genomics products and services include the use of custom synthesized microarrays based on the µParaflo® on-chip synthesis technology which enables the total customization of content on each individual microarray to suit your needs. In addition, the flexibility of µParaflo® enables the incorporation of modifications within the probes, which significantly expands the range of applications for these microarrays. Listed below are applications of custom microarrays containing non-standard probes along with selected references. 
| Microarray Type | Application Examples | Reference |
| DNA oligonucleotides |
|
1 |
| RNA oligonucleotides |
|
2,3 |
| Phosphorothioate oligonucleotides |
|
4 |
| Duplex DNA sequences |
|
5 |
| Modified oligonucleotides |
|
6 |
| 5’- or 3’-labeling |
|
|
| 5’ to 3’ reverse synthesis |
|
7 |
- Tian, J., Gong, H., Sheng, N., Zhou, X., Gulari, E., Gao, X., and Church, G. (2004) Accurate multiplex gene synthesis from programmable DNA chips.Nature 432, 1050-1054. [abstract]
- (a) Burgstaller, P., Jenne, A., and Blind, M. (2002) Aptamers and aptazymes: accelerating small molecule drug discovery. Curr. Opin. Drug Discov. Devel. 5, 690-700. (b) Murphy, MB., Fuller, S. T., Richardson, P. M., and Doyle, S. A. (2003) An improved method for the in vitro evolution of aptamers and applications in protein detection and purification. Nucleic Acids Res. 31, e110.
- (a) Tolstrup, N., Nielsen, P. S., Kolberg, J. G., Frankel, A. M., Vissing, H., and Kauppinen, S. (2003) OligoDesign: Optimal design of LNA (locked nucleic acid) oligonucleotide capture probes for gene expression profiling. Nucleic Acids Res. 31, 3758-3762. (b) Vester, B., and Wengel, J. (2004) LNA (locked nucleic acid): high-affinity targeting of complementary RNA and DNA. Biochemistry 43, 13233-13241.
- Bassett, S. E., Fennewald, S.M., King, D.J., Li, X., Herzog, N. K., Shope, R., Aronson, J. F., Luxon, B. A., and Gorenstein, D. G. (2004) Combinatorial selection and edited combinatorial selection of phosphorothioate aptamers targeting human nuclear factor-kappaB RelA/p50 and RelA/RelA. Biochemistry 43, 9105-9015.
- (a) Bulyk, M. L., Huang, X., Choo, Y., and Church, G. M. (2001) Exploring the DNA-binding specificities of zinc fingers with DNA microarrays. Proc. Natl. Acad. Sci. USA 98, 7158-7163. (b) Mukherjee, S., Berger, M. F., Jona, G., Wang, X. S., Muzzey, D., Snyder, M., Young, R. A., and Bulyk, M. L. (2004) Rapid analysis of the DNA-binding specificities of transcription factors with DNA microarrays. Nat. Genet. 36, 1331-1339.
- (a) Harborth, J., Elbashir, S. M., Vandenburgh, K., Manninga, H., Scaringe, S. A., Weber, K., and Tuschl, T. (2003) Sequence, chemical, and structural variation of small interfering RNAs and short hairpin RNAs and the effect on mammalian gene silencing. Antisense Nucleic Acid Drug Dev. 13, 83-105. (b) Grunweller, A., Wyszko, E., Bieber, B., Jahnel, R., Erdmann, V.A., and Kurreck, J. (2003) Comparison of different antisense strategies in mammalian cells using locked nucleic acids, 2′-O-methyl RNA, phosphorothioates and small interfering RNA. Nucleic Acids Res. 31, 3185-3193.
- Hirschhorn, J. N., Sklar, P., Lindblad-Toh, K., Lim, Y. M., Ruiz-Gutierrez, M., Bolk, S., Langhorst, B., Schaffner, S., Winchester, E., and Lander, E. S. (2000) SBE-TAGS: an array-based method for efficient single-nucleotide polymorphism genotyping. Proc. Natl. Acad. Sci. USA 97, 12164-12169.
